Consiglio Nazionale delle Ricerche

Istituto di Bioscienze e BioRisorse

Identification and characterization of proteins and enzymes (Cazymes), especially those from organisms growing in extreme environments for applications in Green Chemistry, Synthetic Biology, and Sustainable Bioenergy.

Area of Research

  • Identification, biochemical characterization and modification of Carbohydrate Active Enzymes (CAZymes), especially from (hyper)thermophilic microorganisms, for their exploitation in lignocellulosic biomasses and waste products hydrolysis and valorisation
  • Isolation, identification, purification and biochemical characterization of protein molecules from different natural sources, including cold-adapted proteins from polar organisms.
  • Innovative biocatalysts involved the carbon cycle for CO2 sequestration processes and the production of valuable four-carbon compounds
  • Functional characterization of nutrient-sensing mechanisms in marine diatoms for biomass production
  • Use of the recombinant DNA technology for the heterologous overexpression of enzymes as well as their immobilisation to the Escherichia coli outer membrane
  • Enzymes  involved in DNA replication, maintenance  and repair: analysis, characterization  and their possible biotechnological applications, pointing the gaze to biosensors

Expertise and Main Technologies

  • Biochemistry, Enzymology, Molecular Biology, Microbiology and Biotechnology
  • Discovery and biochemical/biophysical characterization of proteins and enzymes from different sources, including extremophilic organisms
  • Protein expression and purification; protein engineering
  • Protein-protein interaction analysis by Surface Plasmon Resonance (SPR) and BioLayer Interferometry (BLI) techniques
  • Chemo-enzymatic reaction for biosensor-derived immobilization and innovative product syntheses

Key Organisms

Archea

Bacteria

Diatoms

Industrial Applications

  • Valorisation of different waste products, including lignocellulosic biomasses, for the synthesis of new molecules with high-added value 
  • Use of innovative thermostable protein tags for enzymatic immobilization in biosensors and synthesis of products with high-added value

Industrial Sector

Bulk & Fine Chemicals

Energy

Food Ingredients

Material & Textiles

Pharma

Scientific References

Angela
Capaccio

Researcher

Clemente
Capasso

Researcher

Vincenzo
Carginale

researcher

Maria Antonietta Ciardiello

researcher

Stefania
Crispi

RESEARCHER

Beatrice
Cobucci Ponzano

national node Coordinator

Nicola
Curci

RESEARCHER

Mariarosaria
De Falco

RESEARCHER

Mariarita
De Felice

RESEARCHER

Federica
De Lise

RESEARCHER

Mauro
Di Fenza

infrastructure manager

Stefania
Filosa

RESEARCHER

Daniela
Giordano

RESEARCHER

Alessandra
Rogato

RESEARCHER

Maurizio
Tamburrini

RESEARCHER

Cinzia
Verde

RESEARCHER

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