Consiglio Nazionale delle Ricerche - CNR | IBBR

Consiglio Nazionale delle Ricerche - CNR | IBBR

Identification and characterization of proteins and enzymes (Cazymes), especially those from organisms growing in extreme environments for applications in Green Chemistry, Synthetic Biology, and Sustainable Bioenergy

Area of Research

  • Identification, biochemical characterization and modification of Carbohydrate Active Enzymes (CAZymes), especially from (hyper)thermophilic microorganisms, for their exploitation in lignocellulosic biomasses and waste products hydrolysis and valorisation
  • Isolation, identification, purification and biochemical characterization of protein molecules from different natural sources, including cold-adapted proteins from polar organisms.
  • Innovative biocatalysts involved the carbon cycle for CO2 sequestration processes and the production of valuable four-carbon compounds
  • Functional characterization of nutrient-sensing mechanisms in marine diatoms for biomass production
  • Use of the recombinant DNA technology for the heterologous overexpression of enzymes as well as their immobilisation to the Escherichia coli outer membrane
  • Enzymes  involved in DNA replication, maintenance  and repair: analysis, characterization  and their possible biotechnological applications, pointing the gaze to biosensors

Area of Expertise and Main Technologies

  • Biochemistry, Enzymology, Molecular Biology, Microbiology and Biotechnology
  • Discovery and biochemical/biophysical characterization of proteins and enzymes from different sources, including extremophilic organisms
  • Protein expression and purification; protein engineering
  • Protein-protein interaction analysis by Surface Plasmon Resonance (SPR) and BioLayer Interferometry (BLI) techniques
  • Chemo-enzymatic reaction for biosensor-derived immobilization and innovative product syntheses

Key Organisms

  • Archaea 
  • Bacteria
  • Diatoms

Industrial Application

  • Valorisation of different waste products, including lignocellulosic biomasses, for the synthesis of new molecules with high added value 
  • Use of innovative thermostable protein tags for enzymatic immobilization in biosensors and synthesis of products with high added value

Industrial Sector

  • Bulk & Fine Chemicals 
  • Energy
  • Food Ingredients
  • Material & Textiles
  • Pharma



Main contact: Beatrice Cobucci-Ponzano -

Clemente Capasso -

Vincenzo Carginale -

Maria Antonietta Ciardiello -

Stefania Crispi - 

Mariarosaria De Falco -

Mariarita De Felice -

Stefania Filosa - 

Daniela Giordano -

Giuseppe Perugino -

Alessandra Rogato -

Maurizio Tamburrini -

Modification date : 19 March 2024 | Publication date : 18 April 2022 | Redactor : Mauro Di Fenza